A Novel Diagnostic Method for Malaria Using Loop Mediated Isothermal Amplification (LAMP) and MinION Nanopore Sequencer
نویسندگان
چکیده
منابع مشابه
A Novel Diagnostic Method for Malaria Using Loop Mediated Isothermal Amplification (LAMP) and MinION Nanopore Sequencer
BACKGROUND A simple and accurate molecular diagnostic method for malaria is urgently needed due to the limitations of conventional microscopic examination. In this study, we demonstrate a new diagnostic procedure for human malaria using loop mediated isothermal amplification (LAMP) and the MinION™ nanopore sequencer. METHODS We generated specific LAMP primers targeting the 18S-rRNA gene of al...
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Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...
متن کاملDetermination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran
Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...
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The loop-mediated isothermal amplification method (LAMP) is a recently developed molecular technique that amplifies nucleic acid under isothermal conditions. For malaria diagnosis, 150 blood samples from consecutive febrile malaria patients, and healthy subjects were screened in Thailand. Each sample was diagnosed by LAMP, microscopy and nested polymerase chain reaction (nPCR), using nPCR as th...
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ژورنال
عنوان ژورنال: Open Forum Infectious Diseases
سال: 2017
ISSN: 2328-8957
DOI: 10.1093/ofid/ofx163.1618